Honda Laboratory





Environmental Sources and Transmission of Nontuberculous Mycobacteria.
Honda JR.Clin Chest Med. 2023 Dec;44(4):661-674. doi: 10.1016/j.ccm.2023.07.001. Epub 2023 Aug 23.PMID: 37890909 Review.


Complete genome assembly of Hawai'i environmental nontuberculous mycobacteria reveals unexpected co-isolation with methylobacteria.
Hendrix J, Epperson LE, Tong EI, Chan YL, Hasan NA, Dawrs SN, Norton GJ, Virdi R, Crooks JL, Chan ED, Honda JR, Strong M.PLoS One. 2023 Sep 13;18(9):e0291072. doi: 10.1371/journal.pone.0291072. eCollection 2023.PMID: 37703253 Free PMC article.

The Many Hosts of Mycobacteria 9 (MHM9): A conference report.
Klever AM, Alexander KA, Almeida D, Anderson MZ, Ball RL, Beamer G, Boggiatto P, Buikstra JE, Chandler B, Claeys TA, Concha AE, Converse PJ, Derbyshire KM, Dobos KM, Dupnik KM, Endsley JJ, Endsley MA, Fennelly K, Franco-Paredes C, Hagge DA, Hall-Stoodley L, Hayes D Jr, Hirschfeld K, Hofman CA, Honda JR, Hull NM, Kramnik I, Lacourciere K, Lahiri R, Lamont EA, Larsen MH, Lemaire T, Lesellier S, Lee NR, Lowry CA, Mahfooz NS, McMichael TM, Merling MR, Miller MA, Nagajyothi JF, Nelson E, Nuermberger EL, Pena MT, Perea C, Podell BK, Pyle CJ, Quinn FD, Rajaram MVS, Mejia OR, Rothoff M, Sago SA, Salvador LCM, Simonson AW, Spencer JS, Sreevatsan S, Subbian S, Sunstrum J, Tobin DM, Vijayan KKV, Wright CTO, Robinson RT.Tuberculosis (Edinb). 2023 Sep;142:102377. doi: 10.1016/ Epub 2023 Jul 23.PMID: 37531864 Review.

Immunological and metabolic characterization of environmental Mycobacterium chimaera infection in a murine model.

Dawrs SN, Virdi R, Islam MN, Hasan NA, Norton GJ, Crooks JL, Parr J, Heinz D, Cool CD, Belisle JT, Chan ED, Honda JR.Microbes Infect. 2023 Jul 14:105184. doi: 10.1016/j.micinf.2023.105184. Online ahead of print.PMID: 37453489 Free article.

Mercaldo RA, Marshall JE, Cangelosi GA, Donohue M, Falkinham JO 3rd, Fierer N, French JP, Gebert MJ, Honda JR, Lipner EM, Marras TK, Morimoto K, Salfinger M, Stout J, Thomson R, Prevots DR. Environmental risk of nontuberculous mycobacterial infection: Strategies for advancing methodology. Tuberculosis (Edinb). 2023 Mar;139:102305. doi: 10.1016/ Epub 2023 Jan 10. PubMed PMID: 36706504; PubMed Central PMCID: PMC10023322.


Gross JE, Teneback CC, Sweet JG, Caceres SM, Poch KR, Hasan NA, Jia F, Epperson LE, Lipner EM, Vang CK, Honda JR, Strand MJ, Calado Nogueira de Moura V, Daley CL, Strong M, Davidson RM, Nick JA. Molecular Epidemiologic Investigation of Mycobacterium intracellulare subspecies chimaera Lung Infections at an Adult Cystic Fibrosis Program. Ann Am Thorac Soc. 2023 Jan 19;. doi: 10.1513/AnnalsATS.202209-779OC. [Epub ahead of print] PubMed PMID: 36656594.


Lipner EM, French JP, Nelson S, Falkinham Iii JO, Mercaldo RA, Blakney RA, Daida YG, Frankland TB, Messier KP, Honda JR, Honda S, Prevots DR. Vanadium in groundwater aquifers increases the risk of MAC pulmonary infection in O'ahu, Hawai'i. Environ Epidemiol. 2022 Oct;6(5):e220. doi: 10.1097/EE9.0000000000000220. eCollection 2022 Oct. PubMed PMID: 36249270; PubMed Central PMCID: PMC9555944.


Parsons AW, Dawrs SN, Nelson ST, Norton GJ, Virdi R, Hasan NA, Epperson LE, Holst B, Chan ED, Leos-Barajas V, Reich BJ, Crooks JL, Strong M, Pacifici K, Honda JR. Soil Properties and Moisture Synergistically Influence Nontuberculous Mycobacterial Prevalence in Natural Environments of Hawai'i. Appl Environ Microbiol. 2022 May 10;88(9):e0001822. doi: 10.1128/aem.00018-22. Epub 2022 Apr 18. PubMed PMID: 35435715; PubMed Central PMCID: PMC9088257.


Gross JE, Caceres S, Poch K, Hasan NA, Jia F, Epperson LE, Lipner E, Vang C, Honda JR, Strand M, Calado Nogueira de Moura V, Daley CL, Strong M, Davidson RM, Nick JA. Investigating Nontuberculous Mycobacteria Transmission at the Colorado Adult Cystic Fibrosis Program. Am J Respir Crit Care Med. 2022 May 1;205(9):1064-1074. doi: 10.1164/rccm.202108-1911OC. PubMed PMID: 35085056; PubMed Central PMCID: PMC9851486.


Vang CK, Dawrs SN, Oberlag NM, Gilmore AE, Hasan NA, Honda JR. Comparative survival of environmental and clinical Mycobacterium abscessus isolates in a variety of diverse host cells. J Appl Microbiol. 2022 Apr;132(4):3302-3314. doi: 10.1111/jam.15416. Epub 2022 Jan 4. PubMed PMID: 34919308; PubMed Central PMCID: PMC9306708.


Fifor A, Krukowski K, Honda JR. Sex, ancestry, senescence, and aging (SAnSA) are stark drivers of nontuberculous mycobacterial pulmonary disease. J Clin Tuberc Other Mycobact Dis. 2022 Feb;26:100297. doi: 10.1016/j.jctube.2022.100297. eCollection 2022 Feb. Review. PubMed PMID: 35059508; PubMed Central PMCID: PMC8760511.


Gross JE, Caceres S, Poch K, Hasan NA, Davidson RM, Epperson LE, Lipner E, Vang C, Honda JR, Strand M, Strong M, Saiman L, Prevots DR, Olivier KN, Nick JA. Healthcare-associated links in transmission of nontuberculous mycobacteria among people with cystic fibrosis (HALT NTM) study: Rationale and study design. PLoS One. 2021;16(12):e0261628. doi: 10.1371/journal.pone.0261628. eCollection 2021. PubMed PMID: 34929010; PubMed Central PMCID: PMC8687591.


Nelson ST, Robinson S, Rey K, Brown L, Jones N, Dawrs SN, Virdi R, Norton GJ, Epperson LE, Hasan NA, Chan ED, Strong M, Honda JR. Exposure Pathways of Nontuberculous Mycobacteria Through Soil, Streams, and Groundwater, Hawai'i, USA. Geohealth. 2021 Apr;5(4):e2020GH000350. doi: 10.1029/2020GH000350. eCollection 2021 Apr. PubMed PMID: 33855248; PubMed Central PMCID: PMC8025848.


Hasan NA, Norton GJ, Virdi R, Epperson LE, Vang CK, Hellbusch B, Bai X, Chan ED, Strong M, Honda JR. Measurable genomic changes in Mycobacterium avium subsp. hominissuis after long-term adaptation in Acanthamoeba lenticulata and reduced persistence in macrophages. J Bacteriol. 2021 Mar 15;203(6). doi: 10.1128/JB.00257-20. Epub 2021 Jan 11. PubMed PMID: 33431432; PubMed Central PMCID: PMC8095452.


Virdi R, Lowe ME, Norton GJ, Dawrs SN, Hasan NA, Epperson LE, Glickman CM, Chan ED, Strong M, Crooks JL, Honda JR. Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai'i Environmental Water Biofilms Compared to Indoor Samples. Microorganisms. 2021 Jan 22;9(2). doi: 10.3390/microorganisms9020224. PubMed PMID: 33499212; PubMed Central PMCID: PMC7910870.


Honda JR, Chan YL, Tong EI, Kawatachi M, Virdi R, Norton GJ, Dawrs SN, Hasan NA, Epperson LE, Crooks JT, Chan ED, Strong M. Inaugural nontuberculous mycobacterial lung disease education and research conference, Honolulu, Hawai'i, February 1-2, 2020. Microbes Infect. 2021 Jan-Feb;23(1):104763. doi: 10.1016/j.micinf.2020.09.009. Epub 2020 Oct 6. PubMed PMID: 33035706; PubMed Central PMCID: PMC7536519.


Hendrix J, Epperson LE, Durbin D, Honda JR, Strong M. Intraspecies plasmid and genomic variation of Mycobacterium kubicae revealed by the complete genome sequences of two clinical isolates. Microb Genom. 2021 Jan;7(1). doi: 10.1099/mgen.0.000497. Epub 2020 Dec 23. PubMed PMID: 33355531; PubMed Central PMCID: PMC8115904.


Tran T, Dawrs SN, Norton GJ, Virdi R, Honda JR. Brought to you courtesy of the red, white, and blue-pigments of nontuberculous mycobacteria. AIMS Microbiol. 2020;6(4):434-450. doi: 10.3934/microbiol.2020026. eCollection 2020. PubMed PMID: 33364537; PubMed Central PMCID: PMC7755587.


Glickman CM, Virdi R, Hasan NA, Epperson LE, Brown L, Dawrs SN, Crooks JL, Chan ED, Strong M, Nelson ST, Honda JR. Assessment of Soil Features on the Growth of Environmental Nontuberculous Mycobacterial Isolates from Hawai'i. Appl Environ Microbiol. 2020 Oct 15;86(21). doi: 10.1128/AEM.00121-20. Print 2020 Oct 15. PubMed PMID: 32859599; PubMed Central PMCID: PMC7580544.


Honda JR. Reply to Ward et al.: Clonally Related Viable Nontuberculous Mycobacteria in Gastric Juice and Sputum in People with Cystic Fibrosis. Am J Respir Crit Care Med. 2020 Oct 1;202(7):1062. doi: 10.1164/rccm.202005-1802LE. PubMed PMID: 32521170; PubMed Central PMCID: PMC7528803.


Dawrs SN, Kautz M, Chan ED, Honda JR. Mycobacteriumabscessus and Gastroesophageal Reflux: An In Vitro Study. Am J Respir Crit Care Med. 2020 Aug 1;202(3):466-469. doi: 10.1164/rccm.202001-0011LE. PubMed PMID: 32298605.


Norton GJ, Williams M, Falkinham JO 3rd, Honda JR. Physical Measures to Reduce Exposure to Tap Water-Associated Nontuberculous Mycobacteria. Front Public Health. 2020;8:190. doi: 10.3389/fpubh.2020.00190. eCollection 2020. PubMed PMID: 32596197; PubMed Central PMCID: PMC7304319.


Honda JR, Hess T, Carlson R, Kandasamy P, Nieto Ramirez LM, Norton GJ, Virdi R, Islam MN, Mehaffy C, Hasan NA, Epperson LE, Hesser D, Alper S, Strong M, Flores SC, Voelker DR, Dobos KM, Chan ED. Nontuberculous Mycobacteria Show Differential Infectivity and Use Phospholipids to Antagonize LL-37. Am J Respir Cell Mol Biol. 2020 Mar;62(3):354-363. doi: 10.1165/rcmb.2018-0278OC. PubMed PMID: 31545652; PubMed Central PMCID: PMC7055699.


Feng Z, Bai X, Wang T, Garcia C, Bai A, Li L, Honda JR, Nie X, Chan ED. Differential Responses by Human Macrophages to Infection With Mycobacterium tuberculosis and Non-tuberculous Mycobacteria. Front Microbiol. 2020;11:116. doi: 10.3389/fmicb.2020.00116. eCollection 2020. PubMed PMID: 32117140; PubMed Central PMCID: PMC7018682.


Bai X, Bai A, Honda JR, Eichstaedt C, Musheyev A, Feng Z, Huitt G, Harbeck R, Kosmider B, Sandhaus RA, Chan ED. Alpha-1-Antitrypsin Enhances Primary Human Macrophage Immunity Against Non-tuberculous Mycobacteria. Front Immunol. 2019;10:1417. doi: 10.3389/fimmu.2019.01417. eCollection 2019. PubMed PMID: 31293581; PubMed Central PMCID: PMC6606736.


Tran T, Bonham AJ, Chan ED, Honda JR. A paucity of knowledge regarding nontuberculous mycobacterial lipids compared to the tubercle bacillus. Tuberculosis (Edinb). 2019 Mar;115:96-107. doi: 10.1016/ Epub 2019 Feb 28. Review. PubMed PMID: 30948183.


Gebert MJ, Delgado-Baquerizo M, Oliverio AM, Webster TM, Nichols LM, Honda JR, Chan ED, Adjemian J, Dunn RR, Fierer N. Ecological Analyses of Mycobacteria in Showerhead Biofilms and Their Relevance to Human Health. mBio. 2018 Oct 30;9(5). doi: 10.1128/mBio.01614-18. PubMed PMID: 30377276; PubMed Central PMCID: PMC6212831.


Honda JR, Alper S, Bai X, Chan ED. Acquired and genetic host susceptibility factors and microbial pathogenic factors that predispose to nontuberculous mycobacterial infections. Curr Opin Immunol. 2018 Oct;54:66-73. doi: 10.1016/j.coi.2018.06.001. Epub 2018 Jun 21. Review. PubMed PMID: 29936307.


Honda JR, Virdi R, Chan ED. Global Environmental Nontuberculous Mycobacteria and Their Contemporaneous Man-Made and Natural Niches. Front Microbiol. 2018;9:2029. doi: 10.3389/fmicb.2018.02029. eCollection 2018. Review. PubMed PMID: 30214436; PubMed Central PMCID: PMC6125357.


Honda JR, Bai X, Chan ED. Elucidating the Pathogenesis of Nontuberculous Mycobacterial Lung Disease: Lesson from the Six Blind Men and the Elephant. Am J Respir Cell Mol Biol. 2018 Feb;58(2):142-143. doi: 10.1165/rcmb.2017-0317ED. PubMed PMID: 29388833.


Malcolm KC, Caceres SM, Honda JR, Davidson RM, Epperson LE, Strong M, Chan ED, Nick JA. Mycobacterium abscessus Displays Fitness for Fomite Transmission. Appl Environ Microbiol. 2017 Oct 1;83(19). doi: 10.1128/AEM.00562-17. Print 2017 Oct 1. PubMed PMID: 28754702; PubMed Central PMCID: PMC5601336.


Zhao X, Epperson LE, Hasan NA, Honda JR, Chan ED, Strong M, Walter ND, Davidson RM. Complete Genome Sequence of Mycobacterium avium subsp. hominissuis Strain H87 Isolated from an Indoor Water Sample. Genome Announc. 2017 Apr 20;5(16). doi: 10.1128/genomeA.00189-17. PubMed PMID: 28428297; PubMed Central PMCID: PMC5399256.


Adjemian J, Frankland TB, Daida YG, Honda JR, Olivier KN, Zelazny A, Honda S, Prevots DR. Epidemiology of Nontuberculous Mycobacterial Lung Disease and Tuberculosis, Hawaii, USA. Emerg Infect Dis. 2017 Mar;23(3):439-447. doi: 10.3201/eid2303.161827. PubMed PMID: 28221128; PubMed Central PMCID: PMC5382761.


Honda JR, Hasan NA, Davidson RM, Williams MD, Epperson LE, Reynolds PR, Smith T, Iakhiaeva E, Bankowski MJ, Wallace RJ Jr, Chan ED, Falkinham JO 3rd, Strong M. Correction: Environmental Nontuberculous Mycobacteria in the Hawaiian Islands. PLoS Negl Trop Dis. 2016 Dec;10(12):e0005200. doi: 10.1371/journal.pntd.0005200. eCollection 2016 Dec. PubMed PMID: 27911953; PubMed Central PMCID: PMC5135035.


Philley JV, DeGroote MA, Honda JR, Chan MM, Kasperbauer S, Walter ND, Chan ED. Treatment of Non-Tuberculous Mycobacterial Lung Disease. Curr Treat Options Infect Dis. 2016 Dec;8(4):275-296. doi: 10.1007/s40506-016-0086-4. Epub 2016 Oct 11. PubMed PMID: 28529461; PubMed Central PMCID: PMC5436303.


Hasan NA, Honda JR, Davidson RM, Epperson LE, Bankowski MJ, Chan ED, Strong M. Complete Genome Sequence of Mycobacterium chimaera Strain AH16. Genome Announc. 2016 Nov 23;4(6). doi: 10.1128/genomeA.01276-16. PubMed PMID: 27881537; PubMed Central PMCID: PMC5122679.


Honda JR, Hasan NA, Davidson RM, Williams MD, Epperson LE, Reynolds PR, Smith T, Iakhiaeva E, Bankowski MJ, Wallace RJ Jr, Chan ED, Falkinham JO 3rd, Strong M. Environmental Nontuberculous Mycobacteria in the Hawaiian Islands. PLoS Negl Trop Dis. 2016 Oct;10(10):e0005068. doi: 10.1371/journal.pntd.0005068. eCollection 2016 Oct. PubMed PMID: 27780201; PubMed Central PMCID: PMC5079566.


Bai X, Oberley-Deegan RE, Bai A, Ovrutsky AR, Kinney WH, Weaver M, Zhang G, Honda JR, Chan ED. Curcumin enhances human macrophage control of Mycobacterium tuberculosis infection. Respirology. 2016 Jul;21(5):951-7. doi: 10.1111/resp.12762. Epub 2016 Mar 24. PubMed PMID: 27012592.


Abe J, Alop-Mabuti A, Burger P, Button J, Ellsberry M, Hitzeman J, Morgenstern D, Nunies K, Strother M, Darling-Munson J, Chan YL, Cassady R, Vasconcellos SM, Iseman MD, Chan ED, Honda JR. Comparing the temporal colonization and microbial diversity of showerhead biofilms in Hawai'i and Colorado. FEMS Microbiol Lett. 2016 Feb;363(4). doi: 10.1093/femsle/fnw005. Epub 2016 Jan 12. PubMed PMID: 26764424.


Honda JR, Hess T, Malcolm KC, Ovrutsky AR, Bai X, Irani VR, Dobos KM, Chan ED, Flores SC. Pathogenic nontuberculous mycobacteria resist and inactivate cathelicidin: implication of a novel role for polar mycobacterial lipids. PLoS One. 2015;10(5):e0126994. doi: 10.1371/journal.pone.0126994. eCollection 2015. PubMed PMID: 25993058; PubMed Central PMCID: PMC4436335.


Bishwakarma R, Kinney WH, Honda JR, Mya J, Strand MJ, Gangavelli A, Bai X, Ordway DJ, Iseman MD, Chan ED. Epidemiologic link between tuberculosis and cigarette/biomass smoke exposure: Limitations despite the vast literature. Respirology. 2015 May;20(4):556-68. doi: 10.1111/resp.12515. Epub 2015 Mar 25. Review. PubMed PMID: 25808744.


Bai X, Shang S, Henao-Tamayo M, Basaraba RJ, Ovrutsky AR, Matsuda JL, Takeda K, Chan MM, Dakhama A, Kinney WH, Trostel J, Bai A, Honda JR, Achcar R, Hartney J, Joosten LA, Kim SH, Orme I, Dinarello CA, Ordway DJ, Chan ED. Human IL-32 expression protects mice against a hypervirulent strain of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):5111-6. doi: 10.1073/pnas.1424302112. Epub 2015 Mar 27. PubMed PMID: 25820174; PubMed Central PMCID: PMC4413346.


Honda JR, Knight V, Chan ED. Pathogenesis and risk factors for nontuberculous mycobacterial lung disease. Clin Chest Med. 2015 Mar;36(1):1-11. doi: 10.1016/j.ccm.2014.10.001. Epub 2014 Nov 6. Review. PubMed PMID: 25676515.


Bai X, Kinney WH, Su WL, Bai A, Ovrutsky AR, Honda JR, Netea MG, Henao-Tamayo M, Ordway DJ, Dinarello CA, Chan ED. Caspase-3-independent apoptotic pathways contribute to interleukin-32γ-mediated control of Mycobacterium tuberculosis infection in THP-1 cells. BMC Microbiol. 2015 Feb 21;15:39. doi: 10.1186/s12866-015-0366-z. PubMed PMID: 25887904; PubMed Central PMCID: PMC4349755.


Honda JR, Bernhard JN, Chan ED. Natural disasters and nontuberculous mycobacteria: a recipe for increased disease?. Chest. 2015 Feb;147(2):304-308. doi: 10.1378/chest.14-0974. PubMed PMID: 25644904; PubMed Central PMCID: PMC4502755.


Honda JR, Wallace RJ Jr, Chan ED. First you have to row a little boat. Respirology. 2015 Feb;20(2):181-2. doi: 10.1111/resp.12465. Epub 2015 Jan 7. PubMed PMID: 25581353.


Chan ED, Kinney WH, Honda JR, Bishwakarma R, Gangavelli A, Mya J, Bai X, Ordway DJ. Tobacco exposure and susceptibility to tuberculosis: is there a smoking gun?. Tuberculosis (Edinb). 2014 Dec;94(6):544-50. doi: 10.1016/ Epub 2014 Sep 28. Review. PubMed PMID: 25305002.


Kim E, Kinney WH, Ovrutsky AR, Vo D, Bai X, Honda JR, Marx G, Peck E, Lindberg L, Falkinham JO 3rd, May RM, Chan ED. A surface with a biomimetic micropattern reduces colonization of Mycobacterium abscessus. FEMS Microbiol Lett. 2014 Nov;360(1):17-22. doi: 10.1111/1574-6968.12587. Epub 2014 Sep 9. PubMed PMID: 25155501.


Honda JR, Connick E, MaWhinney S, Chan ED, Flores SC. Plasma LL-37 correlates with vitamin D and is reduced in human immunodeficiency virus-1 infected individuals not receiving antiretroviral therapy. J Med Microbiol. 2014 Jul;63(Pt 7):997-1003. doi: 10.1099/jmm.0.070888-0. Epub 2014 May 12. PubMed PMID: 24821067; PubMed Central PMCID: PMC4064354.


Bai X, Feldman NE, Chmura K, Ovrutsky AR, Su WL, Griffin L, Pyeon D, McGibney MT, Strand MJ, Numata M, Murakami S, Gaido L, Honda JR, Kinney WH, Oberley-Deegan RE, Voelker DR, Ordway DJ, Chan ED. Inhibition of nuclear factor-kappa B activation decreases survival of Mycobacterium tuberculosis in human macrophages. PLoS One. 2013;8(4):e61925. doi: 10.1371/journal.pone.0061925. Print 2013. PubMed PMID: 23634218; PubMed Central PMCID: PMC3636238.


Bai X, Ovrutsky AR, Kartalija M, Chmura K, Kamali A, Honda JR, Oberley-Deegan RE, Dinarello CA, Crapo JD, Chang LY, Chan ED. IL-32 expression in the airway epithelial cells of patients with Mycobacterium avium complex lung disease. Int Immunol. 2011 Nov;23(11):679-91. doi: 10.1093/intimm/dxr075. Epub 2011 Oct 27. PubMed PMID: 22033195; PubMed Central PMCID: PMC3203680.


Honda JR, Shang S, Shanley CA, Caraway ML, Henao-Tamayo M, Chan ED, Basaraba RJ, Orme IM, Ordway DJ, Flores SC. Immune Responses of HIV-1 Tat Transgenic Mice to Mycobacterium Tuberculosis W-Beijing SA161. Open AIDS J. 2011;5:86-95. doi: 10.2174/1874613601105010086. Epub 2011 Oct 19. PubMed PMID: 22046211; PubMed Central PMCID: PMC3204420.


Marecki JC, Cota-Gomez A, Vaitaitis GM, Honda JR, Porntadavity S, St Clair DK, Flores SC. HIV-1 Tat regulates the SOD2 basal promoter by altering Sp1/Sp3 binding activity. Free Radic Biol Med. 2004 Sep 15;37(6):869-80. doi: 10.1016/j.freeradbiomed.2004.06.016. PubMed PMID: 15706661.





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